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ACTH Fluorescent EIA Kit

ACTH Fluorescent EIA Kit (Mouse or Rat)(FEK-001-21) is designed for in-vitro quantitative determination of  adrenocorticotropic hormone (ACTH) amounts in rat or mouse samples.

More details

FEK-001-21

96 wells

£ 480.00

ACTH Fluorescent EIA Kit

Specificity : Mouse, Rat
Sensitivity : 28.3 pg/ml
Linear Range: 28.3-400 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
ACTH Fluorescent EIA Kit (mouse and rat) can be used to measure mouse or rat adrenocorticotropic hormone (ACTH) concentrations in serum, plasma, culture media, tissue homogenate, CSF, urine or any biological fluid samples.

Assay Principle:
1. ACTH Fluorescent EIA (mouse or rat) has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined using this by extrapolation from a standard curve.

Cross Reactivity:
ACTH (Rat, Mouse) 100%.
ACTH (Human) 100%.
ACTH (18-39) (Human) 100%.
ACTH (1-24) (Human) 0.01%.
ACTH (7-38) (Human) 0%.
Alpha-MSH 0%.
Beta-Endorphin (Rat) 0%.
PACAP-38 (Ovine)  0%
LH-RH  0%
CRF (Human, Rat) 0%,
Met-Enkephalin 0%.
Alpha-ANP (1-28) (Human, Canine) 0%.
BNP-32 (Human) 0%.

Background:
Adrenocorticotropic hormone (ACTH or corticotropin) is a polypeptide hormone produced and secreted by the pituitary gland in response to the release of another hormone, called corticotropin-releasing hormone (CRH), by the hypothalamus. It is found to act through the stimulation of cell surface. It is made up of 39 amino acids, the first 13 of which may be cleaved to form α-melanocyte-stimulating hormone (α-MSH). Receptors are primarily located on the adrenocortical cells. Released in bursts, so its levels in the blood can vary from minute to minute. It is secreted in several intermittent pulses during the day into the bloodstream and transported around the body. It also increases production of the chemical compounds that trigger an increase in other hormones such as adrenaline and noradrenaline. When cortisol levels rise, ACTH levels normally fall.  When cortisol levels fall, ACTH levels normally rise.

References
1. J Gastroenterol. (2007) 42 (7): 522-7. Stress maladjustment in the pathoetiology of ulcerative colitis. Kuroki et al.
2. Ann N Y Acad Sci. (1999) 885: 268-76. The Role of Proopiomelanocortin-Derived Peptides in Skin Fibroblast and Mast Cell Functions. Teofoli et al.
3. Traffic. (2007) 8 (7): 867-82. Rab18 Inhibits Secretory Activity in Neuroendocrine Cells by Interacting with Secretory Granules. Vazquez-Martinez et al.
4. Br J Dermatol. (2006) 155 (5): 910-5. Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours. Kim et al.

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