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ACTH Chemiluminescent EIA Kit Maximize

ACTH Chemiluminescent EIA Kit

ACTH Chemiluminescent EIA Kit (Mouse or Rat) is an in-vitro quantitative method for analysing concentration of rat and mouse  adrenocorticotropic hormone (ACTH).

More details

CEK-001-21

96 wells

£ 480.00

ACTH Chemiluminscent EIA Kit

Specificity : Mouse, Rat
Sensitivity : 28.3 pg/ml
Linear Range: 28.3-400 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution A (7ml)
- Substrate solution B (7ml)
- Biotinylated peptide (1 vial).
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
ACTH Chemiluminescent EIA Kit (mouse and rat) can be used to measure mouse or rat adrenocorticotropic hormone (ACTH) concentrations in serum, plasma, culture media, tissue homogenate, CSF, urine or any biological fluid samples.

Assay Principle:
1. ACTH Chemiluminescent EIA (mouse or rat) has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Luminescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
ACTH (Rat, Mouse) 100%.
ACTH (Human) 100%.
ACTH (18-39) (Human) 100%.
ACTH (1-24) (Human) 0.01%.
ACTH (7-38) (Human) 0%.
Alpha-MSH 0%.
Beta-Endorphin (Rat) 0%.
PACAP-38 (Ovine)  0%
LH-RH  0%

Background:
Adrenocorticotropic hormone (ACTH or corticotropin) is found to act through the stimulation of cell surface. It is made up of 39 amino acids, the first 13 of which may be cleaved to form α-melanocyte-stimulating hormone (α-MSH). Receptors are primarily located on the adrenocortical cells. . It is secreted in several intermittent pulses during the day into the bloodstream and transported around the body. Also, released in bursts, so its levels in the blood can vary from minute to minuteIt also increases production of the chemical compounds that trigger an increase in other hormones such as adrenaline and noradrenaline. When cortisol levels rise, ACTH levels normally fall.  When cortisol levels fall, ACTH levels normally rise.

References
1. Ann N Y Acad Sci. (1999) 885: 268-76. The Role of Proopiomelanocortin-Derived Peptides in Skin Fibroblast and Mast Cell Functions. Teofoli et al.
2. J Gastroenterol. (2007) 42 (7): 522-7. Stress maladjustment in the pathoetiology of ulcerative colitis. Kuroki et al.
3. Traffic. (2007) 8 (7): 867-82. Rab18 Inhibits Secretory Activity in Neuroendocrine Cells by Interacting with Secretory Granules. Vazquez-Martinez et al.
4. Br J Dermatol. (2006) 155 (5): 910-5. Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours. Kim et al.

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