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Adrenomedullin (1-50) Fluorescent EIA Kit Maximize

Adrenomedullin (1-50) Fluorescent EIA Kit

Rat Adrenomedullin (1-50) Fluorescent EIA Kit (FEK-010-08) is a quantitative method to determine levels of rat adrenomedullin (1-50).

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FEK-010-08

96 wells

£ 480.00

Rat Adrenomedullin Fluorescent EIA Kit

Specificity : Rat
Sensitivity : 22.9 pg/ml
Linear Range: 22.9-264 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Rat Adrenomedullin (1-50) Fluorescent EIA is designed to determine levels of rat Adrenomedullin (1-50) in serum, plasma, any biological fluid samples, culture media, CSF, tissue homogenate and urine samples.

Assay Principle:
1. Rat Adrenomedullin Fluorescent EIA kit has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined using by extrapolation from a standard curve.

Cross Reactivity:
Adrenomedullin (Rat, Human) 100%.
Adrenomedullin (1-50) (Mouse) 100%.
Pro-ADM N20 (Human, Mouse) 0%.
Amylin (Human) 0%.
Endothelin-1 (ET-1) 0%.

Background:
Adrenomedullin (1-50) is a gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns and is a peptide is associated with pheochromocytoma. It shares moderate structural similarity to the calcitonin family of regulatory peptides (calcitonin, CGRP and amylin).

References
1. J Immunol. (2006) 176 (9): 5548-58. A Critical Role for Adrenomedullin-Calcitonin Receptor-Like Receptor in Regulating Rheumatoid Fibroblast-Like Synoviocyte Apoptosis. Uzan, et al.
2. J Sleep Res. (2006) 15 (1): 89-95. Circulating adrenomedullin in obstructive sleep apnoea. Schulz, et al.
3. Pediatr Res. (2005) 57 (1): 49-55. Adrenomedullin is highly expressed in blood monocytes associated with acute Kawasaki disease: a microarray gene expression study. Nomura, et al.
4. World J Gastroenterol. (2003) 9 (10): 2325-7. Adrenomedullin in cirrhotic and non-cirrhotic portal hypertension. Tahan, et al.
5. J Matern Fetal Neonatal Med. (2013):1-18. Relationship between neonatal adrenomedullin and bilirubin levels. Erdinc K, et al.
6. Reprod Biol Endocrinol. (2011) 9: 111. Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1. Lei Li, et al.
7. Eur J Obstet Gynecol Reprod Biol. (2005) 123 (1): 35-40. Vascular expression of adrenomedullin is increased in Wistar rats during early pregnancy. van der Heijden et al.
8. Reproductive Biology and Endocrinology (2010) 8 :130. Coexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation. Lei Li, et al.
9. Am J Physiol Heart Circ Physiol. (2004) 286 (6) : 2159-68. Expressions of adrenomedullin mRNA and protein in rats with hypobaric hypoxia-induced pulmonary hypertension. Nakanishi, et al.

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