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Adrenomedullin Chemiluminescent EIA Kit Maximize

Adrenomedullin Chemiluminescent EIA Kit

Human Adrenomedullin Chemiluminescent EIA Kit (CEK-010-01) is a method intended to detect amounts of  human adrenomedullin.

More details

CEK-010-01

96 wells

£ 480.00

Human Adrenomedullin Chemiluminescent EIA Kit

Specificity : Human
Sensitivity : 16.6 pg/ml
Linear Range: 16.6-298 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution A (7ml)
- Substrate solution B (7ml)
- Biotinylated peptide (1 vial).
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Human Adrenomedullin Chemiluminescent EIA is a quantitative in-vitro protocol for analysing amounts of human adrenomedullin levels in serum, plasma, culture media, CSF, any biological fluid samples, urine and tissue homogenate.

Assay Principle:
1. Human adrenomedullin Chemiluminescent EIA has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Luminescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Adrenomedullin (Human) 100%.
Adrenomedullin (Mouse, Rat) 100%.
Adrenomedullin (13-52)(Human) 100%.
Adrenomedullin-Gly (Human) 1%.
CGRP II 0%
CGRP 0%.
Endothelin-1 0%.
Amylin 0%.
CNP-22 0%.
alpha-ANP (1-28) 0%.
BNP-32 0%.

Background:
Human adrenomedullin is a peptide associated with pheochromocytoma which shares moderate structural similarity to the calcitonin family of regulatory peptides (calcitonin, CGRP and amylin). It is a gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns.

References
1. Eur J Obstet Gynecol Reprod Biol. (2005) 123 (1): 35-40. Vascular expression of adrenomedullin is increased in Wistar rats during early pregnancy. van der Heijden et al.
2. Reproductive Biology and Endocrinology (2010) 8 :130. Coexpression of adrenomedullin and its receptor component proteins in the reproductive system of the rat during gestation. Lei Li, et al.
3. World J Gastroenterol. (2003) 9 (10): 2325-7. Adrenomedullin in cirrhotic and non-cirrhotic portal hypertension. Tahan, et al.
4. J Matern Fetal Neonatal Med. (2013):1-18. Relationship between neonatal adrenomedullin and bilirubin levels. Erdinc K, et al.
5. Am J Physiol Heart Circ Physiol. (2004) 286 (6): 2159-68. Expressions of adrenomedullin mRNA and protein in rats with hypobaric hypoxia-induced pulmonary hypertension. Nakanishi, et al.
6. Reprod Biol Endocrinol. (2011) 9: 111. Adrenomedullin in rat follicles and corpora lutea: expression, functions and interaction with endothelin-1. Lei Li, et al.
7. J Immunol. (2006) 176 (9): 5548-58. A Critical Role for Adrenomedullin-Calcitonin Receptor-Like Receptor in Regulating Rheumatoid Fibroblast-Like Synoviocyte Apoptosis. Uzan, et al.
8. Pediatr Res. (2005) 57 (1): 49-55. Adrenomedullin is highly expressed in blood monocytes associated with acute Kawasaki disease: a microarray gene expression study. Nomura, et al.
9. J Sleep Res. (2006) 15 (1): 89-95. Circulating adrenomedullin in obstructive sleep apnoea. Schulz, et al.

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