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Adropin (34-76) EIA Kit Maximize

Adropin (34-76) EIA Kit

Adropin (34-76) EIA Kit (Human, Rat, Mouse, Horse)(EK-032-35) can be used to measure quantitative concentrations of adropin (34-76).

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EK-032-35

96 wells

£ 430.00

Adropin (34-76) EIA Kit

Specificity : Human, Mouse, Rat, Horse.
Sensitivity : 0.3 ng/ml
Linear Range: 0.3-8.2 ng/ml
Assay Range : 0.01-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Adropin EIA Kit (Human, Mouse, Rat, Horse) can be used for quantitatively analysing in-vitro concentrations of determination of Adropin (34-76) in serum, plasma, culture media and various other biological fluids samples.

Assay Principle:
1. Adropin EIA Kit has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined using this adropin EIA kit by extrapolation from a standard curve.

Cross Reactivity:
Adropin (34-76) (Human, Rat, Mouse) 100%.
MCH (Rat, Human, Rat) 0%
CRF (Human, Rat, Mouse, Feline, Canine) 0%
GLP-1 (7-36) Amide (Human, Mouse, Rat) 0%
CCK (26-33) (Non-sulfated) (Rat, Human, Mouse) 0%
Apelin-36 (Human) 0%
AGRP (83-132) Amide (Human) 0%
Ghrelin (Human) 0%

Background:
Adropin is a recently identified protein that is responsible for the maintenance of insulin resistance and energy homeostasis,  with a molecular weight of (4999 Da) that is composed of 43 amino acids. It’s functions include regulating lipid, protein and carbohydrate metabolisms through the modulation of glucose-mediated insulin release.

References
1. J Clin Endocrinol Metab. (2012) 97 (10): 3783-91. Low circulating adropin concentrations with obesity and aging correlate with risk factors for metabolic disease and increase after gastric bypass surgery in humans. Butler AA, et al.
2. Cardiovasc Ther. (2013) 31 (3): 174-8. Deficiency of a new protein associated with cardiac syndrome X; called adropin. Celik A, et al.
Circulation (2010) 122 (11): 185-92. Adropin (34-76) is a novel regulator of endothelial function. Lovren F. et al.
3. J Dev Orig Health Dis. (2014) 5 (2): 132-41. Placental lipoprotein lipase DNA methylation levels are associated with gestational diabetes mellitus and maternal and cord blood lipid profiles. Houde AA, et al.
4. Intern Med. (2011) 50 (15): 1523-7. Elevated plasma levels of adropin in heart failure patients. Lian W, et al.
5. Cell Metab. (2008) 8 (6): 468-81. Identification of adropin as a secreted factor linking dietary macronutrient intake with energy homeostasis and lipid metabolism. Kumar KG, et al.

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