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Alarin EIA Kit Maximize

Alarin EIA Kit

Human Alarin EIA Kit (EK-026-34) designed to detect in-vitro quantitative amounts of human alarin present.

More details

EK-026-34

96 wells

£ 430.00

Human Alarin EIA Kit

Specificity : Human
Sensitivity : 0.08 ng/ml
Linear Range: 0.08-0.78 ng/ml
Assay Range : 0.01-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Human Alarin EIA Kit is a quantitative procedure designed to measure in-vitro levels of human alarin using culture media, plasma, serum and various other biological fluids samples.

Assay Principle:
1. Human alarin EIA has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined using this human alarin EIA kit by extrapolation from a standard curve.

Cross Reactivity:
Alarin (Human) 100%.
Alarin (Rat) 0%
Galanin (Mouse, Rat)  0%
VIP (Human, Mouse, Rat)  0%
NPY (Human, Mouse, Rat)  0%
PHI (1-27) (Rat) 0%
CGRP (Rat, Mouse)  0%

Background:
Alarin is a splice variant of the GALP gene which excludes exon 3 and it shares only 5 conserved amino acids at the N-terminal region with GALP. GALP is a neuropeptide that has complex actions on energy balance, producing orexigenic effects. Alarin was discovered in 2006 in human neuroblastic tumors (gangliocytes) and was found to be localized around blood vessels in the skin. It has an important function in the inhibiting neurogenic inflammation that is caused by substance P. There is also evidence to suggest that it can increase the secretion of luteinizing hormone in male mice.

References
1. Neuropeptides (2012) 46: 99-104. Effects of alarin on food intake, body weight and luteinizing hormone secretion in male mice. Fraley GS, et al.
2. Front Neuroendocrinol (2011) 32: 1-9. Galanin-like peptide (GALP) is a hypothalamic regulator of energy homeostasis and reproduction. Lawrence C and Fraley GS.
3. Horm Behav (2005) 48: 141-51. Effects of galanin-like peptide (GALP) on locomotion, reproduction, and body weight in female and male mice. Kauffman AS, et al.
4. J Mol Neurosci (2012) 46:18-32. Distribution of alarin immunoreactivity in the mouse brain. Eberhard N, et al.
5. Neuropeptides (2010) 44: 333-40. Alarin stimulates food intake in male rats and LH secretion in castrated male rats. Van Der Kolk N, et al.
6. Neuropeptides (2013) 47: 37. Alarin 6-25Cys antagonizes alarin-specific effects on food intake and luteinizing hormone secretion. Fraley GS, et al.
7. Proc Natl Acad Sci U S A. (2007) 104 (24): 10217-22. Alarin is a vasoactive peptide. Santic et al.
8. Br J Pharmacol (2010) 161: 601-13. Alarin stimulates food intake and gonadotrophin release in male rats. Boughton CK, et al.

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