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Alarin EIA Kit Maximize

Alarin EIA Kit

Rat Alarin EIA Kit (EK-026-33) is a protocol intended for quantitatively measuring in-vitro levels of rat alarin.

More details

EK-026-33

96 wells

£ 430.00

Rat Alarin EIA Kit

Specificity : Rat
Sensitivity : 0.11 ng/ml
Linear Range: 0.11-2.2 ng/ml
Assay Range : 0.01-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Rat Alarin EIA Kit is designed to be used for quantitatively analysing concentrations of rat alarin in serum, culture media, plasma, serum or various other biological fluids samples.

Assay Principle:
1. Rat alarin EIA has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined using this rat alarin EIA kit by extrapolation from a standard curve.

Cross Reactivity:
Alarin (Rat) 100%.
Alarin (Human) 0%
Galanin - Like Peptide (GALP) (Rat) 0%
Galanin (Mouse, Rat)  0%
VIP (Human, Mouse, Rat)  0%
NPY (Human, Mouse, Rat)  0%
PHI (1-27) (Rat) 0%
CGRP (Rat, Mouse)  0%

Background:
Alarin was discovered in 2006 in human neuroblastic tumors (gangliocytes) and was found to be localized around blood vessels in the skin and is a splice variant of the GALP gene which excludes exon 3 and it shares only 5 conserved amino acids at the N-terminal region with GALP. There is also evidence to suggest that it can increase the secretion of luteinizing hormone in male mice. It has an important function in the inhibiting neurogenic inflammation that is caused by substance P.

References
1. Br J Pharmacol (2010) 161: 601-13. Alarin stimulates food intake and gonadotrophin release in male rats. Boughton CK, et al.
2. J Mol Neurosci (2012) 46:18-32. Distribution of alarin immunoreactivity in the mouse brain. Eberhard N, et al.
3. Neuropeptides (2012) 46: 99-104. Effects of alarin on food intake, body weight and luteinizing hormone secretion in male mice. Fraley GS, et al.
4. Proc Natl Acad Sci U S A. (2007) 104 (24): 10217-22. Alarin is a vasoactive peptide. Santic et al.
5. Horm Behav (2005) 48: 141-51. Effects of galanin-like peptide (GALP) on locomotion, reproduction, and body weight in female and male mice. Kauffman AS, et al.
6. Neuropeptides (2010) 44: 333-40. Alarin stimulates food intake in male rats and LH secretion in castrated male rats. Van Der Kolk N, et al.
7. Front Neuroendocrinol (2011) 32: 1-9. Galanin-like peptide (GALP) is a hypothalamic regulator of energy homeostasis and reproduction. Lawrence C and Fraley GS.
8. Neuropeptides (2013) 47: 37. Alarin 6-25Cys antagonizes alarin-specific effects on food intake and luteinizing hormone secretion. Fraley GS, et al.

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