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Alarin Fluorescent EIA Kit Maximize

Alarin Fluorescent EIA Kit

Human Alarin Fluorescent EIA Kit (FEK-026-34) can be utilised to detect quantitative concentrations of human alarin.

More details

FEK-026-34

96 wells

£ 480.00

Human Alarin Fluorescent EIA Kit

Specificity : Human
Sensitivity : 8.2 pg/ml
Linear Range: 8.2-115 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Human Alarin Fluorescent EIA kit can be used to measure levels of human alarin in plasma, serum, any biological fluid samples, CSF and urine.

Assay Principle:
1. Human alarin fluorescent EIA has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Background:
Alarin was discovered in 2006 in human neuroblastic tumors (gangliocytes) and was found to be localized around blood vessels in the skin and is a splice variant of the GALP gene which excludes exon 3 and it shares only 5 conserved amino acids at the N-terminal region with GALP. It has an important function in the inhibiting neurogenic inflammation that is caused by substance P. There is also evidence to suggest that it can increase the secretion of luteinizing hormone in male mice.

References
1. Neuropeptides (2012) 46: 99-104. Effects of alarin on food intake, body weight and luteinizing hormone secretion in male mice. Fraley GS, et al.
2. Br J Pharmacol (2010) 161: 601-13. Alarin stimulates food intake and gonadotrophin release in male rats. Boughton CK, et al.
3. Horm Behav (2005) 48: 141-51. Effects of galanin-like peptide (GALP) on locomotion, reproduction, and body weight in female and male mice. Kauffman AS, et al.
4. Front Neuroendocrinol (2011) 32: 1-9. Galanin-like peptide (GALP) is a hypothalamic regulator of energy homeostasis and reproduction. Lawrence C and Fraley GS.
5. J Mol Neurosci (2012) 46:18-32. Distribution of alarin immunoreactivity in the mouse brain. Eberhard N, et al.
6. Proc Natl Acad Sci U S A. (2007) 104 (24): 10217-22. Alarin is a vasoactive peptide. Santic et al.
7. Neuropeptides (2013) 47: 37. Alarin 6-25Cys antagonizes alarin-specific effects on food intake and luteinizing hormone secretion. Fraley GS, et al.
8. Neuropeptides (2010) 44: 333-40. Alarin stimulates food intake in male rats and LH secretion in castrated male rats. Van Der Kolk N, et al.

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