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Angiotensin I Fluorescent EIA Kit Maximize

Angiotensin I Fluorescent EIA Kit

Angiotensin I Fluorescent EIA Kit (Human, Rat, Mouse, Canine)(FEK-002-01) a protocol for in-vitro quantitatively analysing amounts of  angiotensin-I.

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FEK-002-01

96 wells

£ 480.00

Angiotensin I Fluorescent EIA Kit

Specificity : Human, Rat, Mouse, Canine
Sensitivity : 16.0 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Angiotensin I Fluorescent EIA (human, mouse, rat, canine) is a method designed for quatitatively measuring amounts of angiotensin I using samples of serum, any biological fluid samples, urine, plasma and CSF.

Assay Principle:
1. The microwell plate has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Angiotensin I (Human, Mouse, Rat, Canine)  100%.
[Asn1, Val5, Asn9]-Angiotensin I (Salmon)  100%.
[Val5, Ser9]-Angiotensin I (Fowl)  100%.
[Val5, Asn9]-Angiotensin I (Bullfrog)  100%.
α-ANP  (Canine, Human) 0%
Angiotensin II  (Human, Rat, Canine, Mouse) 0%.
Angiotensin III  (Human) 0%.
BNP-32  (Human) 0%.
Renin Substrate  0%.
Endothelin-1  (Porcine, Human, Canine, Bovine, Mouse, Rat) 0%.
[Arg8]-Vasopressin  0%.

Background:
Angiotensin-I has an vital role in the renin-angiotensin system and is found in the blood. It causes increased blood pressure, vasoconstriction and release of aldosterone from the adrenal cortex. It is found to be formed by the action of renin on angiotensinogen. Renin is made by the kidneys in response to both: decreased intra-renal blood pressure at the juxtaglomerular cells and decreased delivery of Na+ and Cl- to the macula densa. It is a powerful dipsogen and it is derived from precursor molecule angiotensinogen, which is a serum globulin that is made in the liver.

References
1. Kidney Int. (2012) 82 (8): 857-66. Endothelial cell TGF-β receptor activation causes tacrolimus-induced renal arteriolar hyalinosis. Valorie L. Chiasson.
2. Am J Nephrol. (2008) 28 (1): 8-13. Effects of Resibufogenin in Experimental Hypertension. Danchuck et al.
3. Asean Heart J; (2011) 19: 20-23. Different Plasma Angiotensin I levels in Indonesian Hypertensive Patients. Nani Hersunarti, et al.
4. Am Soc Nephrol. (2011) 22 (7): 1240–1251. Podocyte COX-2 Exacerbates Diabetic Nephropathy by Increasing Podocyte (Pro)renin Receptor Expression. Huifang Cheng et al.

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