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Angiotensin II Fluorescent EIA Kit Maximize

Angiotensin II Fluorescent EIA Kit

Angiotensin II Fluorescent EIA Kit (Human, Mouse, Rat, Canine)(FEK-002-12) a procedure for quantitative measurment of angiotensin-II.

More details

FEK-002-12

96 wells

£ 480.00

Angiotensin II Fluorescent EIA Kit

Specificity : Human, Rat, Mouse, Canine
Sensitivity : 13.0 pg/ml
Linear Range: 13.0-240.0 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Angiotensin II Fluorescent EIA (human, mouse, canine, rat) is a quatitative in-vitro protocol designed to determine amounts angiotensin II in CSF, serum, plasma,  any biological fluid samples and urine.

Assay Principle:
1. The microwell plates have been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Angiotensin II (Human, Mouse, Canine, Rat)  100%.
[Val5]-Angiotensin II (Human)  100%.
Angiotensin III (Human)  100%.
Angiotensin I (Human)  1%.
Angiotensin II (1-7) (Human)  0%.
Angiotensin I (1-9) (Human)  1%.
Renin Substrate  0%.
[Arg8]-Vasopressin 0%.
Angiotensin (1-7)  0%.
α-ANP (1-28)  0%.
[Sar1, Ile8]-Angiotensin II  0%.

Background:
Human Angiotensin-II has prothrombotic potential by its aggregation and adhesion of platelets which is found to act as an autocrine, paracrine, intracrine hormone and endocrine. It can also cause vascular and cardiac muscle hypertrophy. Has a direct effect on the proximal tubules to increase naion resorption and can increase the thirst sensation through the subfornical organ of the brain.

References
1. Life Sci. (2006) 78 (26): 3066-73. Regulation of cardiac and renal mineralocorticoid receptor expression by captopril following myocardial infarction in rats. de Resende et al.
2. Clin Exp Metastasis. (2012) 29 (8): 915-25. Diabetes-associated angiotensin activation enhances liver metastasis of colon cancer. Takasumi Shimomoto, et al.
3. Circulation. (2005) 111 (15): 1954-61. Insulin-mediated upregulation of the renin angiotensin system in human subcutaneous adipocytes is reduced by rosiglitazone. Harte et al.
4. Hypertension. (2006) 48 (6): 1095-102. Local Renin Angiotensin Expression Regulates Human Mesenchymal Stem Cell Differentiation to Adipocytes. Matsushita et al.
5. J Hum Hypertens. (2003) 17 (2): 133-8. Endothelin-1 and Vasopressin plasma levels are not associated with the insertion/deletion polymorphism of the human angiotensin 1-converting enzyme gene in patients with coronary artery disease. Al-Fakhri et al.
6. Hypertension. (2012) 60: 285-287. Role of Copper Transport Protein Antioxidant 1 in Angiotensin II–Induced Hypertension: A Key Regulator of Extracellular Superoxide Dismutase. Kiyoshi Ozumi, et al.
7. Biology of Reproduction. (2012) 86 (3): 68. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Gao H, et al.
8. J Mol Cell Cardiol. (2006) 40 (5): 696-707. Nuclear accumulation of the AT1 receptor in a rat vascular smooth muscle cell line: effects upon signal transduction and cellular proliferation. Cook et al.
9. BMC Pediatr. (2012) 12: 119. Association of body mass index, sagittal abdominal diameter and waist-hip ratio with cardiometabolic risk factors and adipocytokines in arab children and adolescents. Al-Attas OS, et al.
10. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.

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