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Angiotensin II Fluorescent EIA Kit Maximize

Angiotensin II Fluorescent EIA Kit

Angiotensin II Fluorescent EIA Kit (Human, Mouse, Rat, Canine)(FEK-002-12) a procedure for quantitative measurment of angiotensin-II.

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96 wells

£ 480.00

Angiotensin II Fluorescent EIA Kit

Specificity : Human, Rat, Mouse, Canine
Sensitivity : 13.0 pg/ml
Linear Range: 13.0-240.0 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Angiotensin II Fluorescent EIA (human, mouse, canine, rat) is a quatitative in-vitro protocol designed to determine amounts angiotensin II in CSF, serum, plasma,  any biological fluid samples and urine.

Assay Principle:
1. The microwell plates have been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Angiotensin II (Human, Mouse, Canine, Rat)  100%.
[Val5]-Angiotensin II (Human)  100%.
Angiotensin III (Human)  100%.
Angiotensin I (Human)  1%.
Angiotensin II (1-7) (Human)  0%.
Angiotensin I (1-9) (Human)  1%.
Renin Substrate  0%.
[Arg8]-Vasopressin 0%.
Angiotensin (1-7)  0%.
α-ANP (1-28)  0%.
[Sar1, Ile8]-Angiotensin II  0%.

Human Angiotensin-II has prothrombotic potential by its aggregation and adhesion of platelets which is found to act as an autocrine, paracrine, intracrine hormone and endocrine. It can also cause vascular and cardiac muscle hypertrophy. Has a direct effect on the proximal tubules to increase naion resorption and can increase the thirst sensation through the subfornical organ of the brain.

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