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Angiotensin II Chemiluminescent EIA Kit Maximize

Angiotensin II Chemiluminescent EIA Kit

Angiotensin II Chemiluminescent EIA Kit (Human, Rat, Canine, Mouse)(CEK-002-12) is intended to determine quantitatively concentrations of angiotensin-II.

More details

CEK-002-12

96 wells

£ 480.00

Angiotensin II Chemiluminescent EIA Kit

Specificity : Human, Canine, Mouse, Rat
Sensitivity : 5.9 pg/ml
Linear Range: 5.9-162 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution A (7ml)
- Substrate solution B (7ml)
- Biotinylated peptide (1 vial).
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Angiotensin II Chemiluminescent EIA is an invitro  quantitative method designed to measure levels of angiotensin-II in serum, plasma, culture media, CSF, any biological fluid samples, urine and tissue homogenate.

Assay Principle:
1. Microwell plate has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Luminescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Angiotensin II (Human, Mouse, Canine, Rat)  100%.
[Val5]-Angiotensin II (Human)  100%.
Angiotensin III (Human)  100%.
Renin Substrate  0%.
Angiotensin I (Human)  1%.
Angiotensin I (1-9) (Human)  1%.
Angiotensin II (1-7) (Human)  0%.
[Sar1, Ile8]-Angiotensin II  0%.
[Arg8]-Vasopressin 0%.
Angiotensin (1-7)  0%.
α-ANP (1-28)  0%.

Background:
Angiotensin-II is found to act as an autocrine, intracrine hormone, paracrine, or endocrine and it has a prothrombotic potential by its aggregation and adhesion of platelets. Can increase the thirst sensation through the subfornical organ of the brain by its direct effect on the proximal tubules to increase naion resorption.

References
1. Circulation. (2005) 111 (15): 1954-61. Insulin-mediated upregulation of the renin angiotensin system in human subcutaneous adipocytes is reduced by rosiglitazone. Harte et al.
2. BMC Pediatr. (2012) 12: 119. Association of body mass index, sagittal abdominal diameter and waist-hip ratio with cardiometabolic risk factors and adipocytokines in arab children and adolescents. Al-Attas OS, et al.
3. Hypertension. (2006) 48 (6): 1095-102. Local Renin Angiotensin Expression Regulates Human Mesenchymal Stem Cell Differentiation to Adipocytes. Matsushita et al.
4. Clin Exp Metastasis. (2012) 29 (8): 915-25. Diabetes-associated angiotensin activation enhances liver metastasis of colon cancer. Takasumi Shimomoto, et al.
5. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.
6. Hypertension. (2012) 60: 285-287. Role of Copper Transport Protein Antioxidant 1 in Angiotensin II–Induced Hypertension: A Key Regulator of Extracellular Superoxide Dismutase. Kiyoshi Ozumi, et al.
7. J Hum Hypertens. (2003) 17 (2): 133-8. Endothelin-1 and Vasopressin plasma levels are not associated with the insertion/deletion polymorphism of the human angiotensin 1-converting enzyme gene in patients with coronary artery disease. Al-Fakhri et al.
8. Biology of Reproduction. (2012) 86 (3): 68. Protein restriction to pregnant rats increases the plasma levels of angiotensin II and expression of angiotensin II receptors in uterine arteries. Gao H, et al.
9. Life Sci. (2006) 78 (26): 3066-73. Regulation of cardiac and renal mineralocorticoid receptor expression by captopril following myocardial infarction in rats. de Resende et al.
10. J Mol Cell Cardiol. (2006) 40 (5): 696-707. Nuclear accumulation of the AT1 receptor in a rat vascular smooth muscle cell line: effects upon signal transduction and cellular proliferation. Cook et al.

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