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ANP Chemiluminescent EIA Kit Maximize

ANP Chemiluminescent EIA Kit

ANP Chemiluminescent EIA Kit (Mouse, Rat)(CEK-005-24) is a quantitative method to measure concentrations of atrial natriuretic peptide (ANP).

More details

CEK-005-24

96 wells

£ 480.00

ANP Chemiluminescent EIA Kit

Specificity : Mouse, Rat
Sensitivity : 7.4 pg/ml
Linear Range: 7.4-251 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution A (7ml)
- Substrate solution B (7ml)
- Biotinylated peptide (1 vial).
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
ANP Chemiluminescent EIA (rat, mouse) is a procedure intended for quantitatively detecting concentrations of rat or mouse atrial natriuretic peptide (ANP) in serum, plasma, culture media, CSF, any biological fluid samples, urine and tissue homogenate.

Assay Principle:
1. Microwell plate has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Luminescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
ANP (Mouse, Rat) 100%
Beta ANP (1-28)(Human) 100%.
Alpha ANP (1-28)(Human, Canine, Porcine, Bovine) 65.7%.
Alpha ANP (8-33)(Rat) 100%.
Urodilatin/ANP 100%.
BNP-26 (Porcine)  0.1%.
BNP-45 (Rat)  0%.
BNP-32  (Human) 0%.
Endothelin-1 (Human, Porcine, Mouse, Rat, Bovine) 0%.
CNP-22 (Porcine, Rat, Human)  0%.

Background:
Atrial natriuretic peptide (ANP) is a 28 amino acid peptide with a 17 amino acid ring in the middle of the molecule which is vital role in the homeostatic control of body water, adiposity and sodium. It functions to lower the sodium, water and adipose loads on the circulatory system, thereby reducing blood pressure. It is released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure.

References
1. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.
2. Cardiovasc Res. (2007) 75 (1): 40-50. S100A4 is upregulated in injured myocardium and promotes growth and survival of cardiac myocytes. Schneider et al.
3. Endocrinology. (2007) 148 (5): 2075-84. Alteration of Glucose Homeostasis in V1a Vasopressin Receptor-Deficient Mice. Aoyagi et al.
4. Am J Physiol Heart Circ Physiol. (2009) 296 (3): 655-61. Atrial natriuretic peptide increases inflammation, infarct size, and mortality after experimental coronary occlusion. Houng et al.

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