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ANP Fluorescent EIA Kit

ANP Fluorescent EIA Kit (Mouse, Rat)(FEK-005-24) designed to measure quantitative concentrations of atrial natriuretic peptide (ANP).

More details

FEK-005-24

96 wells

£ 480.00

ANP Fluorescent EIA Kit

Specificity : Rat, Mouse
Sensitivity : 10.5 pg/ml
Linear Range: 10.5-760.0 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
ANP Fluorescent EIA kit (rat, mouse) a protocol intended to measure levels of  rat or mouse atrial natriuretic peptide (ANP) in CSF, serum, plasma,  any biological fluid samples and urine.

Assay Principle:
1. The microwell plates have been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
ANP (1-28)(Mouse, Rat) 100%
Beta ANP (1-28)(Human) 100%.
Alpha ANP (1-28)(Human, Canine, Porcine, Bovine) 65.7%.
Alpha ANP (8-33)(Rat) 100%.
Urodilatin/ANP (95-126) 100%.
BNP-26 (Porcine)  0.1%.
BNP-45 (Rat)  0%.
BNP-32  (Human) 0%.
Endothelin-1 (Human, Porcine, Mouse, Rat, Bovine) 0%.
CNP-22 (Porcine, Rat, Human)  0%.

Background:
Atrial natriuretic peptide (ANP) functions to lower the sodium, water and adipose loads on the circulatory system, thereby reducing blood pressure. It is released by atrial myocytes, muscle cells in the atria of the heart, in response to high blood pressure. It is a 28 amino acid peptide with a 17 amino acid ring in the middle of the molecule which is vital role in the homeostatic control of body water, adiposity and sodium. It

References
1. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.
2. Am J Physiol Heart Circ Physiol. (2009) 296 (3): 655-61. Atrial natriuretic peptide increases inflammation, infarct size, and mortality after experimental coronary occlusion. Houng et al.
3. Endocrinology. (2007) 148 (5): 2075-84. Alteration of Glucose Homeostasis in V1a Vasopressin Receptor-Deficient Mice. Aoyagi et al.
4. Cardiovasc Res. (2007) 75 (1): 40-50. S100A4 is upregulated in injured myocardium and promotes growth and survival of cardiac myocytes. Schneider et al.

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