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Apelin-36 Fluorescent EIA Kit Maximize

Apelin-36 Fluorescent EIA Kit

Human Apelin-36 Fluorescent EIA Kit (FEK-057-15) is a quantitative in-vitro protocol for analysing human apelin 36 concentrations.

More details

FEK-057-15

96 wells

£ 480.00

Human Apelin-36 Fluorescent EIA Kit

Specificity : Human
Sensitivity : 20.0 pg/ml
Linear Range: 20.0-480.0 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Human Apelin-36 fluorescent EIA is designed to measure human apelin-36 using samples of plasma, serum, CSF, any biological fluid samples or urine.

Assay Principle:
1. The microwell plates have been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Apelin-36 (Human) 100%.
Apelin-36 (Rat) 100%.
Apelin-13 (Human) 100%.
Apelin-12 (Human) 100%.
ET-1  (Human) 0%.
ANP (25-56) (N-ANP) 0%.
ADM-52 (Human)  0%.
CNP-22 0%.
BNP-32  0%.
Alpha ANP (Human)  0%.
Ghrelin  (Human) 0%.
Bradykinin  0%.

Background:
Apelin 36 levels are increased in left ventricles of patients with chronic heart failure and it known to function as a mediator of the cardiovascular control, which includes blood flow and blood pressure. A potent stimulator of cardiac contractility, playing a vital part in cardiac tissue remodelling which is expressed in various organs such as the brain, heart, lung, kidney, endothelium, gastrointestinal tract , adrenal glands adipose tissue or human plasma.

References
1. In Vivo (2002) 16 (5): 333-6. Apelin expression in normal human tissues. De Falco et al.
2. Peptides. (2012) 37 (2): 233-9. The bioactive peptides salusins and apelin-36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass. Suna Aydin, et al.
3. Steroids. (2012) 77 (13): 1352-8. Serum asymmetric dimethylarginine, apelin, and tumor necrosis factor-α levels in non-obese women with polycystic ovary syndrome. Young Sik Choi, et al.
4. Eur J Endocrinol (2008) 158: 905-910. Effect of hypocaloric diet-induced weight loss in obese women on plasma apelin and adipose tissue expression of apelin and APJ. Isabelle Castan-Laurel et al.,
5. Ren Fail. 2012;34(9):1068-73. Volume Status in Patients on Peritoneal Dialysis: The Role of Apelin and Bio-Impedance Spectroscopy. Rumeyza Kazancioglu, et al.

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