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Apelin-36 EIA Kit Maximize

Apelin-36 EIA Kit

Apelin-36 EIA Kit (Mouse, Rat)(EK-057-28) is intened for in-vitro quantitativemeasurment of rat and mouse apelin 36 concentrations.

More details

EK-057-28

96 wells

£ 480.00

Apelin-36 EIA Kit

Specificity : Rat, Mouse
Sensitivity : 0.26 ng/ml
Linear Range: 0.26-2.0 ng/ml
Assay Range : 0.01-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Apelin-36 EIA (rat or mouse) is an in-vitro quantitative method designed to determine levels of mouse and rat apelin-36 in samples of plasma, serum, culture media and various other biological fluids samples.

Assay Principle:
1.  Pre-coated microwells with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Apelin-36 (Rat) 100%.
Apelin-36 (Human) 100%.
Apelin-12 (Human) 100%.
Apelin-13 (Human) 100%.
Alpha-ANP (Human)  0%.
ADM-52 (Human)  0%.
ANP (25-56) (N-ANP)  0%.
CNP-22 0%.
ET-1  (Human) 0%.
BNP-32 (Human)  0%.
Bradykinin  0%.
Ghrelin  (Human) 0%.

Background:
Apelin 36 levels are increased in left ventricles of patients with chronic heart failure and it known to function as a mediator of the cardiovascular control, which includes blood flow and blood pressure. A potent stimulator of cardiac contractility, playing a vital part in cardiac tissue remodelling which is expressed in various organs such as the brain, heart, lung, kidney, endothelium, gastrointestinal tract , adrenal glands adipose tissue or human plasma.

References
1. Steroids. (2012) 77 (13): 1352-8. Serum asymmetric dimethylarginine, apelin, and tumor necrosis factor-α levels in non-obese women with polycystic ovary syndrome. Young Sik Choi, et al.
2. Ren Fail. 2012;34(9):1068-73. Volume Status in Patients on Peritoneal Dialysis: The Role of Apelin and Bio-Impedance Spectroscopy. Rumeyza Kazancioglu, et al.
3. In Vivo (2002) 16 (5): 333-6. Apelin expression in normal human tissues. De Falco et al.
4. Eur J Endocrinol (2008) 158: 905-910. Effect of hypocaloric diet-induced weight loss in obese women on plasma apelin and adipose tissue expression of apelin and APJ. Isabelle Castan-Laurel et al.,
5. Peptides. (2012) 37 (2): 233-9. The bioactive peptides salusins and apelin-36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass. Suna Aydin, et al.

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