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APL 1beta-28 EIA Kit Maximize

APL 1beta-28 EIA Kit

APL 1beta-28 EIA Kit (EK-018-42) is intended to measure quantitative concentrations of APL 1beta-28 in-vitro.

More details

EK-018-42

96 wells

£ 430.00

APL 1 beta-28 EIA

Sensitivity : 0.29 ng/ml
Linear Range: 0.4-6.4 ng/ml
Assay Range : 0-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
APL 1beta-28 EIA can be used for quantitatively determing in-vitro levels of APL 1beta-28 in samples of plasma, serum, culture media and various other biological fluids samples.

Assay Principle:
1. Pre-coated microwell plate with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
APL 1β-28 100%.
APL 1β-27  72%.
Amyloid b-Protein (1-40) (Human) 0%.
APL 1β-28 (1-14)  0%.
Amyloid b-Protein (1-43)  0%. 
Amyloid b-Protein (1-42)  0%.

Background:
Amyloid-like protein 1 (APLP1) are encoded by the APLP1 gene, this gene is known to contain a conserved amyloid precursor protein gene. There are many spliced transcript variants (isoforms) that have been discovered, these are found to play many different roles in synaptic maturation during cortical development.

References
1. Brain Res. Mol. Brain Res. (1996) 32 (1): 36-44. Selective localization of amyloid precursor-like protein 1 in the cerebral cortex postsynaptic density. Kim TW, et al.
2. J. Pathol. (2008) 215 (2): 155-63. Identification of the Alzheimer's disease amyloid precursor protein (APP) and its homologue APLP2 as essential modulators of glucose and insulin homeostasis and growth. Needham BE, et al.
3. Biochemistry (2002) 41 (30): 9310-20. Evidence for a copper-binding superfamily of the amyloid precursor protein. Simons A, et al.
4. Genomics (1993) 15 (1): 237-9. The amyloid precursor-like protein (APLP) gene maps to the long arm of human chromosome 19. Wasco W, et al.
5. J. Biol. Chem. (2004) 279 (11): 10542-50. Cleavage of amyloid-beta precursor protein and amyloid-beta precursor-like protein by BACE 1. Li Q and Südhof TC.

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