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APL 1beta-28 Fluorescent EIA Kit Maximize

APL 1beta-28 Fluorescent EIA Kit

APL 1beta-28 Fluorescent EIA Kit (FEK-018-42) is an in-vitro quantitative method to determine levels of APL 1beta-28 present.

More details

FEK-018-42

96 wells

£ 480.00

APL 1β-28 Fluorescent EIA Kit

Sensitivity : 51 pg/ml
Linear Range: 51-1125 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
APL 1β-28 fluorescent EIA kit can be used for determining levels of APL 1beta-28 present in samples of CSF, serum, plasma, urine and any biological fluid samples.

Assay Principle:
1. The microwell plates have been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
APL 1β-28 100%.
APL 1β-27  72%.
Amyloid b-Protein (1-40) (Human) 0%.
APL 1β-28 (1-14)  0%.
Amyloid b-Protein (1-43)  0%. 
Amyloid b-Protein (1-42)  0%.

Background:
Amyloid-like protein 1 (APLP1) are encoded by the APLP1 gene, this gene is known to contain a conserved amyloid precursor protein gene. There are many spliced transcript variants (isoforms) that have been discovered, these are found to play many different roles in synaptic maturation during cortical development.

References
1. J. Biol. Chem. (2004) 279 (11): 10542-50. Cleavage of amyloid-beta precursor protein and amyloid-beta precursor-like protein by BACE 1. Li Q and Südhof TC.
2. Brain Res. Mol. Brain Res. (1996) 32 (1): 36-44. Selective localization of amyloid precursor-like protein 1 in the cerebral cortex postsynaptic density. Kim TW, et al.
3. Biochemistry (2002) 41 (30): 9310-20. Evidence for a copper-binding superfamily of the amyloid precursor protein. Simons A, et al.
4. J. Pathol. (2008) 215 (2): 155-63. Identification of the Alzheimer's disease amyloid precursor protein (APP) and its homologue APLP2 as essential modulators of glucose and insulin homeostasis and growth. Needham BE, et al.
5. Genomics (1993) 15 (1): 237-9. The amyloid precursor-like protein (APLP) gene maps to the long arm of human chromosome 19. Wasco W, et al.

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