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Betatropin (133-198) EIA Kit Maximize

Betatropin (133-198) EIA Kit

Mouse Betatropin (133-198) EIA Kit (EK-051-57) can be used to quantitatively detect concentrations of mouse betatropin (133-198).

More details

EK-051-57

96 wells

£ 480.00

Mouse Betatropin (133-198) EIA Kit

Specificity : Mouse
Sensitivity : 0.23 ng/ml
Linear Range: 0.23-4.20 ng/ml
Assay Range : 0-100ng/ml
Size: 96 tests.

Reagents Supplied:
- Primary antibody (rabbit anti-peptide IgG)(1 vial).
- 2N HCl (15ml).
- Standard peptide (1 vial).
- Streptavidin-horseradish peroxidase (SA-HRP)(30μl).
- Substrate solution (TMB) (12ml).
- 96 well immunoplate (1 plate).
- 20x assay buffer concentrate (50ml).
- Positive control (2 vials).
- Acetate plate sealer (APS), (3 pieces).
- Biotinylated peptide (1 vial).
- Assay diagram (1 sheet).
- General protocol (1 book).

Intended Use:
Mouse betatropin (133-198) EIA is an in-vitro quantitative procedure to measure concentrations of mouse betatropin (133-198) using serum, plasma, culture media, CSF and various other biological fluids samples.

Assay Principle:
1. Pre-coated microwell plate with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the TMB substrate solution and hydrogen peroxide to form a blue coloured solution.
4. Reaction is stopped by adding hydrogen chloride, which forms a yellow coloured solution.
5. Intensity of the yellow solution is inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Betatrophin (133-198) (Mouse) 100%.
Betatrophin (131-198) (Rat)  17%.
Betatrophin (84-138) (Human) 0%.
Betatrophin (139-198) (Human) 0%.
Betatrophin (22-83) (Human)  0%.
Betatrophin (Human)  0%.
Neuropeptide Y (Human, Mouse, Rat)  0%.
Irisin, Recombinant (Human, Mouse, Rat, Canine)  0%.
AGRP (83-132)-Amide (Human)  0%.
Insulin (Human)  0%.
Ghrelin (Human)  0%.
PEDF-34 (Human)  0%.

Background:
Betatrophin belongs to the angiopoietin-like gene family, but does not contain the C-terminal fibrinogen-like domain and is a gene which is located on chromosome 19 (humans) and chromosome 9 (mouse). It is also a 22kDa protein which is made of two coiled-coil domains and an N-terminal secretion signal, a peptide hormone which has been shown in mice to elevate the rate of beta cells undergoing cell division.

References
1. Cell (2014) 159 (3): 691-696. ANGPTL8/Betatrophin Does Not Control Pancreatic Beta Cell Expansion. Gusarova V, et al.
2. Biochem Biophys Res Commun. (2012) 424 (4): 786-92. Lipasin, a novel nutritionally-regulated liver-enriched factor that regulates serum triglyceride levels. Zhang R
3. Diabetes (2014) 63 (4): 1198-99. Betatrophin versus bitter-trophin and the elephant in the room: time for a new normal in β-cell regeneration research. Stewart AF.
4. Am J Physiol Endocrinol Metab (2012) 303 (3): 334. Identification of RIFL, a novel adipocyte-enriched insulin target gene with a role in lipid metabolism. Ren G, et al.
5. Proc Natl Acad Sci U S A (2013) 110 (10): 16109-14. Mice lacking ANGPTL8 (Betatrophin) manifest disrupted triglyceride metabolism without impaired glucose homeostasis. Wang Y, et al.
6. Diabetes (2014) 63 (4): 1283-8. Elevated mouse hepatic betatrophin expression does not increase human β-cell replication in the transplant setting. Jiao Y, et al.
7. Cell (2013) 153 (4): 747-58. Betatrophin: A Hormone that Controls Pancreatic β Cell Proliferation. Yi P, et al.

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