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BNP-32 Chemiluminescent EIA Kit Maximize

BNP-32 Chemiluminescent EIA Kit

Porcine BNP-32 Chemiluminescent EIA Kit (CEK-011-10) can be used for quantitatively analysing amounts of porcine brain natriuretic peptide-32 (BNP-32) present.

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CEK-011-10

96 wells

£ 480.00

Porcine BNP-32 Chemiluminescent EIA Kit

Specificity : Porcine
Sensitivity : 12.0 pg/ml
Linear Range: 12.0-535.0 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution A (7ml)
- Substrate solution B (7ml)
- Biotinylated peptide (1 vial).
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Porcine BNP-32 chemiluminescent EIA is a protocol designed to determine in-vitro quantitative amounts of porcine brain natriuretic peptide-32 (BNP-32) in samples of urine, serum, tissue homogenate, plasma, culture media, CSF or any biological fluid.

Assay Principle:
1. Microwell plate has been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
5. Luminescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
6. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
BNP-32 (Porcine)  100%.
BNP-26 (Porcine)  100%.
BNP-32 (Rat, Human)  0%.
BNP-45 (Rat)  0%.
Alpha ANP (1-28)(Canine, Human, Ovine)  0%.

Background:
Brain natriuretic peptide was first discovered in extracts of porcine brain, whereas within humans it is found to be made in the cardiac ventricles. It is a 32 a.a. polypeptide has been found to be secreted by the ventricles of heart when there is excessive stretching of myocytes. It is release alongside an NT-proBNP (76 amino acids amino terminal fragment). Elevated levels of BNP 32 and/or NT-proBNP within the blood are useful markers in the diagnosis of heart failure and acute congestive heart failure (CHF).

References
1. Cardiovasc Pathol. (2007) 16 (2): 79-84. Plasma brain natriuretic peptide correlates with infarct size but not with subsequent remodeling in the rat heart. Maczewski et al.
2. Circulation. (2007) 116 (4): 399-410. ß1-Adrenergic Receptor Autoantibodies Mediate Dilated Cardiomyopathy by Agonistically Inducing Cardiomyocyte Apoptosis. Jane-wit et al.
3. Circulation. (2007) 115 (1): 67-75. Hemodynamic Modulation of Endocardial Thromboresistance. Kapur et al.
4. J Mol Cell Cardiol. (2006) 41 (5): 798-806. Down-regulation of cardiac apelin system in hypertrophied and failing hearts: Possible role of angiotensin II-angiotensin type 1 receptor system. Iwanaga et al.

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