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Alpha MSH Fluorescent EIA Kit Maximize

Alpha MSH Fluorescent EIA Kit

Alpha MSH Fluorescent EIA Kit (Human, Rat, Mouse)(FEK-043-01) can be used to determine quantitative levels of melanocyte stimulating hormone (alpha-MSH).

More details

FEK-043-01

96 wells

£ 480.00

Alpha MSH Fluorescent EIA (Melanocyte Stimulating Hormone)

Specificity : Human, Rat, Mouse.
Sensitivity :20.0 pg/ml
Linear Range: 20.0-384.0 pg/ml
Range: 0-10000 pg/ml
Size: 96 tests

Reagents Supplied:
- 96 well immunoplate (1 plate)
- 20x assay buffer concentrate (50ml)
- Standard peptide (1 vial).
- Primary antibody (rabbit anti-peptide IgG) (1 vial).
- Positive control (1 vial).
- Acetate plate sealer (APS), (3 pieces)
- Streptavidin-horseradish peroxidase (SA-HRP) (30μl)
- Substrate solution (12ml)
- Biotinylated peptide (1 vial).
- Stable peroxide solution (1.5ml)
- Stop solution (12ml)
- General protocol (1 book).
- Assay diagram (1 sheet).

Intended Use:
Alpha MSH fluorescent EIA (human, mouse, rat) is designed to measure in-vitro quantitative concentrations of alpha melanin concentrating hormone (alpha MCH) using samples of  serum, plasma, CSF, culture media, tissue homogenate and any biological fluid samples and urine.

Assay Principle:
1. The microwell plates have been pre-coated with secondary antibody and also the non-specific binding sites have been blocked.
2. Secondary antibody is able to bind with the Fc fragment of the primary antibody, the Fab segment is competitively bound by both biotinylated peptides and the peptide standard or the targeted peptide in samples.
3. Biotinylated peptide can then interact with the SA-HRP, which then catalyzes the substrate solution.
4. Fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples.
5. Unknown concentration in samples can then be determined by extrapolation from a standard curve.

Cross Reactivity:
Alpha MSH (Human, Mouse, Rat) 100%.
Des Ac-alpha-MSH 79%.
alpha-MSH (4-13) (Ac-(Cys4, d-Phe7, Cys10)  16.3%.
AGRP (83-132)-NH2 (Human) 0%.
Leptin (Human)  0%.
Neuropeptide Y (Rat, Human)  0%.
ACTH (Human)  0%.
CART (55-102)(Human)  0%.

Background:
Melanocyte Stimulating Hormones (Alpha-MSH) is an important melanocortin for pigmentation and it functions to stimulate the production/release of melanin by melanocytes in the skin and hair. It belongs to a group called the melanocortins, which also includes ACTH, beta-MSH, beta MSH and alpha MSH. All of these are peptide hormones that are produced by cells in the intermediate lobe of the pituitary gland.

References
1. Br J Dermatol. (2006) 155 (5): 910-5. Investigation of the corticotropin-releasing hormone-proopiomelanocortin axis in various skin tumours. Kim et al.
2. Psychoneuroendocrinology. (2006) 31 (9): 1057-61. Plasma agouti-related protein levels in women with anorexia nervosa. Moriya et al.
3. Anal Biochem. (2003) 316 (1): 34-40. Generation of a bioactive neuropeptide in a cell-free system. Jiang et al.
4. Toxicol Lett. (2009) 185 (3): 168-74. Dermal microdialysis of inflammatory markers induced by aliphatic hydrocarbons in rats. Patlolla et al.
5. Ann N Y Acad Sci. (1999) 885: 268-76. The Role of Proopiomelanocortin-Derived Peptides in Skin Fibroblast and Mast Cell Functions. Teofoli et al.
6. J Gene Med. (2006) 8 (6): 764-72. Alpha-melanocyte-stimulating hormone gene therapy reverses carbon tetrachloride induced liver fibrosis in mice. Lee et al.
7. Photodermatol Photoimmunol Photomed. (2008) 24 (6): 308-13. Effects of Coccoloba uvifera L. on UV-stimulated melanocytes. Silveira et al.
8. Br J Dermatol. (2005) 153 (3): 498-505. FK506 promotes melanocyte and melanoblast growth and creates a favourable milieu for cell migration via keratinocytes: possible mechanisms of how tacrolimus ointment induces repigmentation in patients with vitiligo. Lan et al.
9. Clin Exp Dermatol. (2012) 37 (1): 65-7. Ultraviolet B radiation to the eye induces pigmentation in the epidermis via the activation of the subunit gp91 phox of reduced nicotinamide adenine dinucleotide phosphate oxidase. Hiramoto, K. and Sato, E. F.
10. Carbohydr Res. (2008) 343 (3): 501-11. Photoprotective effects of glucomannan isolated from Candida utilis. Ruszova et al.
11. Dig Dis Sci. (2008) 53 (2): 556-63. Alpha-melanocyte-stimulating hormone gene transfer attenuates inflammation after bile duct ligation in the rat. Wang et al.
12. Biochem Biophys Res Commun. (2004) 322 (1): 153-61. Single injection of naked plasmid encoding alpha-melanocyte-stimulating hormone protects against thioacetamide-induced acute liver failure in mice. Wang et al.

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