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Adenosine Deaminase Assay Kit

Adenosine deaminase (ADA) assay kit (BQ014-EALD) intended to detect ADA enzyme activity in human serum samples.

More details

BQ014-EALD

275 tests

£ 385.00

Adenosine Deaminase Assay Kit [ADA]

Linear range: 0-200 U/L.
Method: Colorimetric, Enzyme (Kinetic)
Wavelength: 550nm
Test Size: 275 tests.

Product Features:
1. Specific assay for ADA with no detectable reaction with other nucleosides.
2. Is not affected by serum bilirubin up to 20mg/dL, hemoglobin up to 200 mg/dL, triglycerides up to 750mg/dL, and ascorbic acid up to 4 mg/dL.
3. Liquid two reagents system, ready-to-use for both manual method and automated chemistry analyzers (Kinetics)

Intended Use:
This kit for adenosine deaminase assay can be used to determine the activity of human ada in serum samples.

Reagents Supplied:
Below is the composition of Reagent 1 (R1) and Reagent 2 (R1) that is provided with this ada assay kit. The adenosine deaminase assay control can be purchased separately.

Reagent composition for the following ADA assay kit (275 tests):
1. Reagent 1 (R1) 50 mL: 50 mM Tris-HCl pH 8.0, 2 mM 4-AA, 0.1 U/mL PNP, 0.2 U/mL XO, 0.6 U/mL Peroxidase, Stablizers.
2. Reagent 2 (R2) 25 mL: 50mM Tris-HCl pH 4.0, 10 mM Adenosine, 2 mM EHSPT.
3. ADA Control 1.0 mL: Adenosine deaminase (bovine liver) and BSA. ADA Control is sold separately. BQ014-EACN.

Linearity:
This enzyme ADA assay kit is linear from 0-200 U/L (r2 >0.99). Please note that if the activity of ADA is greater than 200U/L, then the sample needs to be diluted with saline and the final result obtained will need to be multiplied by the dilution factor.

Precision:
- Intra Assay: CV% less than 4.5%.
- Inter Assay: CV% less than 6%.

Reference Value:
Healthy subjects have ADA activity in the range of 4 – 20 U/L or 66 – 332 nkat/L when using this kit for adenosine deaminase assay purpose. It is recommended that each laboratory should establish its own range of reference values.

Stability
The reagent (R1) provided with this enzyme ADA assay kit is stable for 9 months if stored at 2 – 8 °C in the dark.

References
1. Mem Inst. Oswaldo Cruz (1999) 94(3) 383-386. Adenosine deaminase and guanosine deaminase activities in sera of patients with viral hepatitis. Kallkan A., Bult V., Erel O., Avci S., and Bingol N. K.
2. Thorax (1995) 50: 672-674. Use of adenosine deaminase as a diagnositic tool for tuberculous pleurisy. Burgess LJ, Maritz FJ, Le Roux I, et al.
3. Am. J. Gastroenterol. (1993) 88: 266-271. ADA isoenzymes in liver disease.  Kobayashi F, Ikeda T, Marumo F, Sato C.

  • ADA Assay
    Detailed protocol kit insert for ADA assay (Adenosine Deaminase).
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