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Carbon Dioxide Assay Kit

Carbon dioxide (CO2) assay (BQ007-EAEL) kit is intended for quantitative in vitro enzyme measurement of human CO2  levels in serum or plasma samples.

More details

BQ007-EAEL

300 tests

£ 385.00

Carbon Dioxide Assay Kit [CO2]

Linear range: 1.12-50mmol/L

Method: Colorimetric Enzyme (Kinetic)
Wavelength: 405nm
Test Size: 300 tests.

Product Features:
1. CO2 assay kit is based on two enzyme coupled reactions that result in a reduction in absorbance, this is directly proportional to levels of CO2 present within the measuring sample at 405 or 415nm.
2. Carbon dioxide reagents that are provided are in a ready-to-use format.
3. Interference: Less that 10% at the conc. shown for the following substances; Ascobic Acid (5mg/ml), Bilirubin (40mg/ml), Hemoglobin (200mg/ml), Triglycerides (1000mg/ml), Bilirubin Conjugated (40mg/ml).

Intended Use:
This CO2 assay kit can be utilsed for in vitro quantitative detection of carbon dioxide amounts in the plasma and serum of humans.


Reagents Table:
Below is the composition of CO2 Reagent 1 (R1) that is supplied with this CO2 assay kit. Carbon dioxide assay controls can be purchased separately

Reagents compositins for this CO2 (Carbon dioxide): (300 tests).
A. Reagent R1:   PEPC, MDH, PEP and NADH in buffer.
B. Calibrator: 30mM Sodium Bicarbonate contained in 0.9% saline.
[plus a 0.9% saline is also included that needs to be used as a zero reference].

** Carbon Dioxide Controls can be purchased separately [BQ007-EACN].

Results:
The concentration of the results obtained for this CO2 assay kit will be expressed in units if mmol/L (mEq/L).

Interference:
The following substances normally present in serum produced less than 10% deviation at the listed concentrations when measured using this Carbon dioxide assay kit: bilirubin conjugated at 40 mg/mL, bilirubin at 40 mg/mL, triglycerides at 1000 mg/ml, hemoglobin at 200 mg/ml and ascorbic acid at 5 mg/mL.

Reference Range:
- 22-29mmol/L (plasma and serum samples) for adults.
- 20-28mmol/L (plasma and serum samples) for children or infants.

Reference
1. Clinical Biochemistry, 7th ed., Al Latner, Editor, Saunders, Philadelphia, (1975) p. 399. Contarow and Trumper.
2. Clinical Chemistry, Editors, CV Mosby Company, St. Louis (MO), (1984) p. 1056. LA Kaplan, AJ Pesce.
3. Fundamentals of Clinical Chemistry, W.B. Saunders Co., Philadelphia, (1982) 865. Tietz, N. W. (Ed). 
4. Textbook of Clinical Chemistry, 2nd ed. Philadelphia, PA: WB Saunders, (1994) p. 2181. Burtis CA, Ashwood ER, eds. Tietz.

  • CO2 assay
    Detailed protocol kit insert for CO2 assay (Carbon Dioxide)
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