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Rapid Fecal Occult Blood Test Strip Maximize

Rapid Fecal Occult Blood Test Strip

Fecal Occult Blood Test Strip (FOB)(BQ 002-RTCC) is intended for rapid qualitative detection of human hemoglobin in human fecal specimens.

More details

BQ 002-RTCC

100 tests

£ 250.00

FOB Test Strip

Principle:
- Specimen needs to be collected in the collection tube which already contains sample extraction buffer. Next add this to the rapid FOB test strip device.
- Mixture travels along the membrane by a capillary action and then reacts with anti-h hemoglobin antibody that is coated on the test region.
- If h hemoglobin is present at levels of 50ng/mL or greater, the result is the formation of a coloured band in the test region.
- If there is no h hemoglobin in the sample, the area will remain colourless.
- The sample continues to move to the control area where goat anti-mouse IgG will capture gold antibody conjugate to form a pink to purple colour, indicating the test is working and the result is valid.
- FOB Test kit  is an immunochromatographic sandwich assay.

Results Interpretation:
1. Positive: If two coloured bands are visible within 3 minutes, the test result is positive and valid.
Note: Specimens containing very low levels of h hemoglobin may develop two coloured bands over 10 minutes.
2. Negative: If test area has no coloured band and the control area displays a coloured band, the result is negative and valid.
3. Invalid result: The test result is invalid if a coloured band does not form in the control region. The sample must be retested using a new test device.

Reagents Supplied:
[A]. Fecal Occult Blood Test device: The amount of each coated antigen and/or antibody on the strip is less than 1.0 mg for antigen conjugate and is less than 1.0 mg for goat anti-mouse IgG antibody.
- Rapid FOB strip [Test zone]: contains mice monoclonal anti-hemoglobin antibody.
- Rapid FOB strip [Control zone]: contains goat anti-mouse IgG antibody.
- Rapid FOB strip [Conjugate pad]: contains gold-mice monoclonal anti-hemoglobin antibody conjugate.
[B]. Fecal specimen collection tube: The collection tube contains 2 ml of 50 mM Tris buffer, pH 7.6.
[C]. Instructions for use

Performance Characteristics: Sensitivity of this rapid test FOB strip is 12.5 μg h hemoglobin/g feces or 50 ng/mL h hemoglobin.

Quality Control:
We recommend that a positive control, containing between 50–200 ng/mL h haemoglobin and a negative control, 0 ng/mL h hemoglobin be used with this test strip FOB . Control materials, which are not provided with this test kit, are commercially available.

Background:
- Occult blood in human fecal specimen is associated with gastrointestinal diseases which could result in colorectal cancer if not treated promptly and properly.
- Colorectal cancer is the well known to be the third most common cancer in the world.
- FOB - is normally defined as a blood loss of less than 50 mL/d.

Reference
1. Annual general Meeting (May 1986) Melbourne, Victoria, Australia. Gasteroenterology society of Australia. Detection of fecal occult blood using monoclonal antibodies. Woo. H. and McDonald C.
2. Gastroenterology (1985) Vol. 88 820. Occult blood screening for colorectal carcinoma: a critical review. Simon J.B.
3. Br. J. Cancer, (1984) Vol. 49 141. Immunological detection of fecal occult blood in colorectal cancer. Taranen, M.J., et al.
4. Ann. Elin. Lab. Sci., (1974) Vol. 4 343. Immunochemical confirmation of gastrointestinal bleeding. Adams, E.C. and Layman, K.M.
5. Lancet, (1980) Vol. 1, 417.  Occult-blood test and colorectal tumors. Ribet, A., et al.

Please contact us if you require any further information on this Rapid Fecal Occult Blood Test

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